• Western blot analysis of extracts of various cell lines, using BAG2 antibody (STJ11103631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using BAG2 Rabbit monoclonal antibody (STJ11103631) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using BAG2 rabbit monoclonal antibody (STJ11103631) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using BAG2 Rabbit monoclonal antibody (STJ11103631) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using BAG2 rabbit monoclonal antibody (STJ11103631) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using BAG2 Rabbit monoclonal antibody (STJ11103631) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using BAG2 rabbit monoclonal antibody (STJ11103631) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using BAG2 antibody (STJ11103631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-BAG2 antibody (1-100) [S1MR] (STJ11103631)

SKU:
STJ11103631

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-BAG2 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAG2
Gene ID: 9532
Uniprot ID: BAG2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BAG2 (O95816).
Immunogen Sequence: MAQAKINAKANEGRFCRSSS MADRSSRLLESLDQLELRVE ALREAATAVEQEKEILLEMI HSIQNSQDMRQISDGEREEL NLTANRLMGRTLTVEVSVET
Function Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.
Protein Name Bag Family Molecular Chaperone Regulator 2
Bag-2
Bcl-2-Associated Athanogene 2
Database Links Reactome: R-HSA-3371453
Alternative Antibody Names Anti-Bag Family Molecular Chaperone Regulator 2 antibody
Anti-Bag-2 antibody
Anti-Bcl-2-Associated Athanogene 2 antibody
Anti-BAG2 antibody

Information sourced from Uniprot.org

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