![Western blot analysis of lysates from wild type (WT) and B4GALT1 knockout (KO) HeLa cells, using [KO Validated] B4GALT1 Rabbit pAb (STJ111285) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/91703/408543/STJ111285_1__83305.1744894851.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and B4GALT1 knockout (KO) HeLa cells, using [KO Validated] B4GALT1 Rabbit pAb (STJ111285) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and B4GALT1 knockout (KO) HeLa cells, using [KO Validated] B4GALT1 Rabbit pAb (STJ111285) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/91703/408543/STJ111285_1__83305.1744894851.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and B4GALT1 knockout (KO) HeLa cells, using [KO Validated] B4GALT1 Rabbit pAb (STJ111285) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
Anti-B4GALT1 antibody (45-210) (STJ111285)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal B4GALT1 (45-210) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | B4GALT1 |
| Gene ID | 2683 |
| Uniprot ID | B4GT1_HUMAN |
| Immunogen Region | 45-210 |
| Immunogen Sequence | RDLSRLPQLVGVSTPLQGGS NSAAAIGQSSGELRTGGARP PPPLGASSQPRPGGDSSPVV DSGPGPASNLTSVPVPHTTA LSLPACPEESPLLVGPMLIE FNMPVDLELVAKQNPNVKMG GRYAPRDCVSPHKVAIIIPF RNRQEHLKYWLYYLHPVLQR QQLDYG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 45-210 of human B4GALT1 (NP_001488.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed, but at very low levels in fetal and adult brain. |
| Post Translational Modifications | The soluble form derives from the membrane forms by proteolytic processing. |
| Function | Beta-1,4-galactosyltransferase 1: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Processed beta-1,4-galactosyltransferase 1: The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. |
| Protein Name | Beta-1 -4-Galactosyltransferase 1Beta-1 -4-Galtase 1Beta4gal-T1B4gal-T1Beta-N-Acetylglucosaminyl-Glycolipid Beta-1 -4-GalactosyltransferaseBeta-N-Acetylglucosaminylglycopeptide Beta-1 -4-GalactosyltransferaseLactose Synthase A ProteinN-Acetyllactosamine SynthaseNal SynthaseNeolactotriaosylceramide Beta-1 -4-GalactosyltransferaseUdp-Gal -Beta-Glcnac Beta-1 -4-Galactosyltransferase 1Udp-Galactose -Beta-N-Acetylglucosamine Beta-1 -4-Galactosyltransferase 1 Cleaved Into - Processed Beta-1 -4-Galactosyltransferase 1 |
| Database Links | Reactome: R-HSA-1912420Reactome: R-HSA-2022854Reactome: R-HSA-2534343Reactome: R-HSA-3656244Reactome: R-HSA-4793953Reactome: R-HSA-5653890Reactome: R-HSA-6798695Reactome: R-HSA-975577 |
| Cellular Localisation | Isoform Long: Golgi ApparatusGolgi Stack MembraneSingle-Pass Type Ii Membrane ProteinCell MembraneCell SurfaceCell ProjectionFilopodiumFound In Trans Cisternae Of Golgi But Is Mainly Localized At The Plasma MembraneB4galt1 Cell Surface Expression Is Regulated By Ube2q1Isoform Short: Golgi ApparatusFound In Trans Cisternae Of GolgiProcessed Beta-14-Galactosyltransferase 1: SecretedSoluble Form Found In Body Fluids |
| Alternative Antibody Names | Anti-Beta-1 -4-Galactosyltransferase 1 antibodyAnti-Beta-1 -4-Galtase 1 antibodyAnti-Beta4gal-T1 antibodyAnti-B4gal-T1 antibodyAnti-Beta-N-Acetylglucosaminyl-Glycolipid Beta-1 -4-Galactosyltransferase antibodyAnti-Beta-N-Acetylglucosaminylglycopeptide Beta-1 -4-Galactosyltransferase antibodyAnti-Lactose Synthase A Protein antibodyAnti-N-Acetyllactosamine Synthase antibodyAnti-Nal Synthase antibodyAnti-Neolactotriaosylceramide Beta-1 -4-Galactosyltransferase antibodyAnti-Udp-Gal -Beta-Glcnac Beta-1 -4-Galactosyltransferase 1 antibodyAnti-Udp-Galactose -Beta-N-Acetylglucosamine Beta-1 -4-Galactosyltransferase 1 Cleaved Into - Processed Beta-1 -4-Galactosyltransferase 1 antibodyAnti-B4GALT1 antibodyAnti-GGTB2 antibody |
Information sourced from Uniprot.org