• Immunofluorescence analysis of PC-12 cells using AXIN2 Rabbit polyclonal antibody (STJ26209) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of AXIN2 in paraffin-embedded mouse spinal cord using AXIN2 Rabbit polyclonal antibody (STJ26209) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AXIN2 in paraffin-embedded rat brain using AXIN2 Rabbit polyclonal antibody (STJ26209) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using AXIN2 Rabbit polyclonal antibody (STJ26209) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) : A-431. Exposure time: 180s.

Anti-AXIN2 antibody (743-843) (STJ26209)

SKU:
STJ26209

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AXIN2 (743-843) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AXIN2
Gene ID: 8313
Uniprot ID: AXIN2_HUMAN
Immunogen Region: 743-843
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 743-843 of human AXIN2 (NP_004646.3).
Immunogen Sequence: APEDHKEPKKLAGVHALQAS ELVVTYFFCGEEIPYRRMLK AQSLTLGHFKEQLSKKGNYR YYFKKASDEFACGAVFEEIW EDETVLPMYEGRILGKVERI D
Tissue Specificity Expressed in brain and lymphoblast.
Post Translational Modifications Probably phosphorylated by GSK3B and dephosphorylated by PP2A. ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
Function Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
Protein Name Axin-2
Axin-Like Protein
Axil
Axis Inhibition Protein 2
Conductin
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-4411364
Reactome: R-HSA-4641257
Reactome: R-HSA-5689880
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Axin-2 antibody
Anti-Axin-Like Protein antibody
Anti-Axil antibody
Anti-Axis Inhibition Protein 2 antibody
Anti-Conductin antibody
Anti-AXIN2 antibody

Information sourced from Uniprot.org

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