| Host: | Rabbit |
| Applications: | WB/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Axin-1 (190-270 aa) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | AXIN1 |
| Gene ID: | 8312 |
| Uniprot ID: | AXIN1_HUMAN |
| Immunogen Region: | 190-270 aa |
| Specificity: | AXIN1 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 190-270 |
| Post Translational Modifications | Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1. ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway. Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. Ubiquitination by SIAH1 and SIAH2 induces its proteasomal degradation as part of the activation of the Wnt signaling pathway. |
| Function | Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects.down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also a component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. |
| Protein Name | Axin-1Axis Inhibition Protein 1Haxin |
| Database Links | Reactome: R-HSA-195253Reactome: R-HSA-196299Reactome: R-HSA-201681Reactome: R-HSA-4641257Reactome: R-HSA-4641262Reactome: R-HSA-5339716Reactome: R-HSA-5358747Reactome: R-HSA-5358749Reactome: R-HSA-5358751Reactome: R-HSA-5358752Reactome: R-HSA-5467337Reactome: R-HSA-5467340Reactome: R-HSA-5467348Reactome: R-HSA-5689880Reactome: R-HSA-8931987Reactome: R-HSA-8939256Reactome: R-HSA-9018519 |
| Cellular Localisation | CytoplasmNucleusMembraneCell MembraneMacf1 Is Required For Its Translocation To Cell MembraneOn Uv IrradiationTranslocates To The Nucleus And Colocalizes With Daax |
| Alternative Antibody Names | Anti-Axin-1 antibodyAnti-Axis Inhibition Protein 1 antibodyAnti-Haxin antibodyAnti-AXIN1 antibodyAnti-AXIN antibody |
Information sourced from Uniprot.org
