| Host: |
Goat |
| Applications: |
WB |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-Ataxin 3 (120-250aa) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:250-1:2000 |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: |
ATXN3 |
| Gene ID: |
4287 |
| Uniprot ID: |
ATX3_HUMAN |
| Immunogen Region: |
120-250aa |
| Accession Number: |
ENSG00000066427 |
| Specificity: |
Detects levels of ATXN3 protein in transfected cells with GFP-ATXN3 and endogenous levels in U118, SH-SY5Y, At-T20 and hTERT cell lysates by Western blot. |
| Immunogen: |
Purified recombinant peptide derived from within residues 120 aa to 250 aa of human ATXN3 produced in E. coli. |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Monoubiquitinated N-terminally by UBE2W, possibly leading to activate the deubiquitinating enzyme activity. |
| Function | Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins. Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription. Acts as a negative regulator of mTORC1 signaling in response to amino acid deprivation by mediating deubiquitination of RHEB, thereby promoting RHEB inactivation by the TSC-TBC complex. Regulates autophagy via the deubiquitination of 'Lys-402' of BECN1 leading to the stabilization of BECN1. |
| Protein Name | Ataxin-3Machado-Joseph Disease Protein 1Spinocerebellar Ataxia Type 3 Protein |
| Database Links | Reactome: R-HSA-5689877Reactome: R-HSA-9615017 |
| Cellular Localisation | Nucleus MatrixNucleusLysosome MembranePeripheral Membrane ProteinPredominantly NuclearBut Not ExclusivelyInner Nuclear MatrixRecruited To Lysosomal Membrane In Response To Amino Acid Deprivation By The Raga/Rraga-Ragb/Rragb Complex |
| Alternative Antibody Names | Anti-Ataxin-3 antibodyAnti-Machado-Joseph Disease Protein 1 antibodyAnti-Spinocerebellar Ataxia Type 3 Protein antibodyAnti-ATXN3 antibodyAnti-ATX3 antibodyAnti-MJD antibodyAnti-MJD1 antibodyAnti-SCA3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
