• Immunofluorescence analysis of U2OS cells using Ataxin-3 (ATXN3) Rabbit polyclonal antibody (STJ22739) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Ataxin-3 (ATXN3) Rabbit polyclonal antibody (STJ22739) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of U-2 OS cells using Ataxin-3 (ATXN3) Polyclonal Antibody (STJ22739) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Ataxin-3 (ATXN3) Rabbit polyclonal antibody (STJ22739) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using Ataxin-3 (ATXN3) Rabbit polyclonal antibody (STJ22739) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Ataxin-3 (ATXN3) Rabbit polyclonal antibody (STJ22739) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Ataxin-3 (ATXN3) antibody (STJ22739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ATXN3 antibody (1-361) (STJ22739)

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STJ22739

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ataxin 3 (1-361) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATXN3
Gene ID: 4287
Uniprot ID: ATX3_HUMAN
Immunogen Region: 1-361
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-361 of human Ataxin-3 (ATXN3) (NP_004984.2).
Immunogen Sequence: MESIFHEKQEGSLCAQHCLN NLLQGEYFSPVELSSIAHQL DEEERMRMAEGGVTSEDYRT FLQQPSGNMDDSGFFSIQVI SNALKVWGLELILFNSPEYQ RLRIDPINERSFICNYKEHW FTVRKLGKQWFNLNSLLTGP ELISDTYLALFLAQLQQEGY SIFVVKGDLPDCEADQLLQM IRVQQMHRPKLIGEELAQLK EQRVHKTDLERVLEANDGSG MLDEDEEDLQRALALSRQE
Tissue Specificity Ubiquitous.
Post Translational Modifications Monoubiquitinated N-terminally by UBE2W, possibly leading to activate the deubiquitinating enzyme activity.
Function Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins. Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription. Acts as a negative regulator of mTORC1 signaling in response to amino acid deprivation by mediating deubiquitination of RHEB, thereby promoting RHEB inactivation by the TSC-TBC complex. Regulates autophagy via the deubiquitination of 'Lys-402' of BECN1 leading to the stabilization of BECN1.
Protein Name Ataxin-3
Machado-Joseph Disease Protein 1
Spinocerebellar Ataxia Type 3 Protein
Database Links Reactome: R-HSA-5689877
Reactome: R-HSA-9615017
Cellular Localisation Nucleus Matrix
Nucleus
Lysosome Membrane
Peripheral Membrane Protein
Predominantly Nuclear
But Not Exclusively
Inner Nuclear Matrix
Recruited To Lysosomal Membrane In Response To Amino Acid Deprivation By The Raga/Rraga-Ragb/Rragb Complex
Alternative Antibody Names Anti-Ataxin-3 antibody
Anti-Machado-Joseph Disease Protein 1 antibody
Anti-Spinocerebellar Ataxia Type 3 Protein antibody
Anti-ATXN3 antibody
Anti-ATX3 antibody
Anti-MJD antibody
Anti-MJD1 antibody
Anti-SCA3 antibody

Information sourced from Uniprot.org

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