STJ72311 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ATXN2 antibody (Internal) (STJ72311)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ72311-100
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General Information

Short DescriptionGoat polyclonal antibody anti-ATXN2 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman/Mouse/Dog/Cow/Pig
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:2000.
IF: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of A431 cells. Recommended concentration

Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolATXN2
Gene ID6311
Uniprot IDATX2_HUMAN
Accession NumberNP_002964.3; NP_001297050.1; NP_001297052.1
Immunogen RegionInternal
Immunogen SequenceSEREGHSINTR

Additional Info

Function Involved in EGFR trafficking, acting as negative regulator of endocytic EGFR internalization at the plasma membrane.
Protein Name Ataxin-2
Spinocerebellar Ataxia Type 2 Protein
Trinucleotide Repeat-Containing Gene 13 Protein
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ataxin-2 antibody
Anti-Spinocerebellar Ataxia Type 2 Protein antibody
Anti-Trinucleotide Repeat-Containing Gene 13 Protein antibody
Anti-ATXN2 antibody
Anti-ATX2 antibody
Anti-SCA2 antibody
Anti-TNRC13 antibody

Information sourced from Uniprot.org

Citations

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