Anti-ATXN1 antibody (562-693 aa) [R01-8H1] (STJA0010993)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human Ataxin 1
General Information
| Short Description | Rabbit monoclonal anti-Ataxin 1 (562-693 aa) for use in WB and IP in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R01-8H1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | ATXN1 |
| Gene ID | 6310 |
| Uniprot ID | ATX1_HUMAN |
| Immunogen | Recombinant protein of human Ataxin 1 |
| Immunogen Region | 562-693 aa |
Additional Info
| Tissue Specificity | Widely expressed throughout the body. |
| Post Translational Modifications | Ubiquitinated by UBE3A, leading to its degradation by the proteasome. The presence of expanded poly-Gln repeats in spinocerebellar ataxia 1 (SCA1) patients impairs ubiquitination and degradation, leading to accumulation of ATXN1 in neurons and subsequent toxicity. Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract. Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract. |
| Function | Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. In concert with CIC and ATXN1L, involved in brain development. |
| Protein Name | Ataxin-1Spinocerebellar Ataxia Type 1 Protein |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusColocalizes With Usp7 In The Nucleus |
| Alternative Antibody Names | Anti-Ataxin-1 antibodyAnti-Spinocerebellar Ataxia Type 1 Protein antibodyAnti-ATXN1 antibodyAnti-ATX1 antibodyAnti-SCA1 antibody |
Information sourced from Uniprot.org