| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ATR (400-500) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATR |
| Gene ID: |
545 |
| Uniprot ID: |
ATR_HUMAN |
| Immunogen Region: |
400-500 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human ATR (NP_001175.2). |
| Immunogen Sequence: |
LKMESMEIIEEIQCQTQQEN LSSNSDGISPKRRRLSSSLN PSKRAPKQTEEIKHVDMNQK SILWSALKQKAESLQISLEY SGLKNPVIEMLEGIAVVLQL T |
| Tissue Specificity | Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary. |
| Post Translational Modifications | Phosphorylated.autophosphorylates in vitro. |
| Function | Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence ST-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex. |
| Protein Name | Serine/Threonine-Protein Kinase AtrAtaxia Telangiectasia And Rad3-Related ProteinFrap-Related Protein 1 |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-176187Reactome: R-HSA-3371453Reactome: R-HSA-5685938Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6783310Reactome: R-HSA-6796648Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9709570 |
| Cellular Localisation | NucleusChromosomeDepending On The Cell TypeIt Can Also Be Found In Pml Nuclear BodiesRecruited To Chromatin During S-PhaseRedistributes To Discrete Nuclear Foci Upon Dna DamageHypoxia Or Replication Fork Stalling |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Atr antibodyAnti-Ataxia Telangiectasia And Rad3-Related Protein antibodyAnti-Frap-Related Protein 1 antibodyAnti-ATR antibodyAnti-FRP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
