| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ATPIF1 (26-106) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:100IP 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ATP5IF1 |
| Gene ID: |
93974 |
| Uniprot ID: |
ATIF1_HUMAN |
| Immunogen Region: |
26-106 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1). |
| Immunogen Sequence: |
GSDQSENVDRGAGSIREAGG AFGKREQAEEERYFRAQSRE QLAALKKHHEEEIVHHKKEI ERLQKEIERHKQKIKMLKHD D |
| Post Translational Modifications | Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid. |
| Function | Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme. |
| Protein Name | Atpase Inhibitor - MitochondrialAtp Synthase F1 Subunit EpsilonInhibitor Of F(1f(O-AtpaseIf(1If1 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Atpase Inhibitor - Mitochondrial antibodyAnti-Atp Synthase F1 Subunit Epsilon antibodyAnti-Inhibitor Of F(1f(O-Atpase antibodyAnti-If(1 antibodyAnti-If1 antibodyAnti-ATP5IF1 antibodyAnti-ATPI antibodyAnti-ATPIF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ATPIF1 knockout (KO) 293T cells, using [KO Validated] ATPIF1 Rabbit polyclonal antibody (STJ11100822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89758/358234/STJ11100822_1__18532.1713122566.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ATPIF1 knockout (KO) 293T cells, using [KO Validated] ATPIF1 Rabbit polyclonal antibody (STJ11100822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
