• Western blot analysis of lysates from HCT116 cells, using ATP8B3 Rabbit polyclonal antibody (STJ11102932) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATP8B3 antibody (600-900) (STJ11102932)

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STJ11102932

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP8B3 (600-900) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP8B3
Gene ID: 148229
Uniprot ID: AT8B3_HUMAN
Immunogen Region: 600-900
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 600-900 of human ATP8B3 (NP_620168.1).
Immunogen Sequence: VTAARNFGYVFLSRTQDTVT IMELGEERVYQVLAIMDFNS TRKRMSVLVRKPEGAICLYT KGADTVIFERLHRRGAMEFA TEEALAAFAQETLRTLCLAY REVAEDIYEDWQQRHQEASL LLQNRAQALQQLLGATAIED RLQDGVPETIKCLKKSNIKI WVLTGDKQETAVNIGFACEL LSENMLILEEKEISRILETY WENSNNLLTRESLSQVKLAL VINGDFLDKLLVSLRKEPR
Tissue Specificity Isoform 3 was only detected in testis.
Function P4-ATPase flippase which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. May be responsible for the maintenance of asymmetric distribution of phosphatidylserine (PS) in spermatozoa membranes. Involved in acrosome reactions and binding of spermatozoa to zona pellucida.
Protein Name Phospholipid-Transporting Atpase Ik
Atpase Class I Type 8b Member 3
Database Links Reactome: R-HSA-936837
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Acrosome Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Phospholipid-Transporting Atpase Ik antibody
Anti-Atpase Class I Type 8b Member 3 antibody
Anti-ATP8B3 antibody
Anti-ATP1K antibody
Anti-FOS37502_2 antibody

Information sourced from Uniprot.org

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