• Immunofluorescence analysis of HeLa cells using ATP6V1E1 Rabbit polyclonal antibody (STJ22732) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody (STJ22732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody (STJ22732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ATP6V1E1 antibody (77-226) (STJ22732)

SKU:
STJ22732

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP6V1E1 (77-226) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP6V1E1
Gene ID: 529
Uniprot ID: VATE1_HUMAN
Immunogen Region: 77-226
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 77-226 of human ATP6V1E1 (NP_001687.1).
Immunogen Sequence: NQARLKVLRARDDLITDLLN EAKQRLSKVVKDTTRYQVLL DGLVLQGLYQLLEPRMIVRC RKQDFPLVKAAVQKAIPMYK IATKNDVDVQIDQESYLPED IAGGVEIYNGDRKIKVSNTL ESRLDLIAQQMMPEVRGALF GANANRKFLD
Tissue Specificity Kidney.localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level). Ubiquitous. High expression in the skin.
Function Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.
Protein Name V-Type Proton Atpase Subunit E 1
V-Atpase Subunit E 1
V-Atpase 31 Kda Subunit
P31
Vacuolar Proton Pump Subunit E 1
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-77387
Reactome: R-HSA-917977
Reactome: R-HSA-9639288
Reactome: R-HSA-983712
Cellular Localisation Apical Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Clathrin-Coated Vesicle Membrane
Alternative Antibody Names Anti-V-Type Proton Atpase Subunit E 1 antibody
Anti-V-Atpase Subunit E 1 antibody
Anti-V-Atpase 31 Kda Subunit antibody
Anti-P31 antibody
Anti-Vacuolar Proton Pump Subunit E 1 antibody
Anti-ATP6V1E1 antibody
Anti-ATP6E antibody
Anti-ATP6E2 antibody

Information sourced from Uniprot.org

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