• Immunofluorescence analysis of L929 cells using ATP5G2 Rabbit polyclonal antibody (STJ11100235) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ATP5G2 Rabbit polyclonal antibody (STJ11100235) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATP5G2 antibody (50-150) (STJ11100235)

SKU:
STJ11100235

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP5G2 (50-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP5MC2
Gene ID: 517
Uniprot ID: AT5G2_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ATP5G2 (NP_001002031.1).
Immunogen Sequence: ATAPHPLKMFACSKFVSTPS LVKSTSQLLSRPLSAVVLKR PEILTDESLSSLAVSCPLTS LVSSRSFQTSAISRDIDTAA KFIGAGAATVGVAGSGAGIG T
Post Translational Modifications Trimethylated by ATPSCKMT at Lys-109. Methylation is required for proper incorporation of the C subunit into the ATP synthase complex and mitochondrial respiration.
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.
Protein Name Atp Synthase F(0 Complex Subunit C2 - Mitochondrial
Atp Synthase Lipid-Binding Protein
Atp Synthase Membrane Subunit C Locus 2
Atp Synthase Proteolipid P2
Atp Synthase Proton-Transporting Mitochondrial F(0 Complex Subunit C2
Atpase Protein 9
Atpase Subunit C
Database Links Reactome: R-HSA-163210
Reactome: R-HSA-8949613
Cellular Localisation Mitochondrion Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp Synthase F(0 Complex Subunit C2 - Mitochondrial antibody
Anti-Atp Synthase Lipid-Binding Protein antibody
Anti-Atp Synthase Membrane Subunit C Locus 2 antibody
Anti-Atp Synthase Proteolipid P2 antibody
Anti-Atp Synthase Proton-Transporting Mitochondrial F(0 Complex Subunit C2 antibody
Anti-Atpase Protein 9 antibody
Anti-Atpase Subunit C antibody
Anti-ATP5MC2 antibody
Anti-ATP5G2 antibody
Anti-PSEC0033 antibody

Information sourced from Uniprot.org

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