Anti-ATP5F1 antibody (1-245) (STJ29959)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATP5F1 (1-245) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATP5PB |
| Gene ID | 515 |
| Uniprot ID | AT5F1_HUMAN |
| Immunogen Region | 1-245 |
| Immunogen Sequence | MLSRVVLSAAATAAPSLKNA AFLGPGVLQATRTFHTGQPH LVPVPPLPEYGGKVRYGLIP EEFFQFLYPKTGVTGPYVLG TGLILYALSKEIYVISAETF TALSVLGVMVYGIKKYGPFV ADFADKLNEQKLAQLEEAKQ ASIQHIQNAIDTEKSQQALV QKRHYLFDVQRNNIAMALEV TYRERLYRVYKEVKNRLDYH ISVQNMMRRKEQEHMINWVE KHVVQSISTQQEKETIAKC |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human ATP5F1 (NP_001679.2). |
Additional Info
| Function | Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. |
| Protein Name | Atp Synthase Peripheral Stalk Subunit B - MitochondrialAtp Synthase F(0 Complex Subunit B1 - MitochondrialAtp Synthase Peripheral Stalk-Membrane Subunit BAtp Synthase Proton-Transporting Mitochondrial F(0 Complex Subunit B1Atp Synthase Subunit BAtpase Subunit B |
| Database Links | Reactome: R-HSA-163210Reactome: R-HSA-8949613 |
| Cellular Localisation | MitochondrionMitochondrion Inner Membrane |
| Alternative Antibody Names | Anti-Atp Synthase Peripheral Stalk Subunit B - Mitochondrial antibodyAnti-Atp Synthase F(0 Complex Subunit B1 - Mitochondrial antibodyAnti-Atp Synthase Peripheral Stalk-Membrane Subunit B antibodyAnti-Atp Synthase Proton-Transporting Mitochondrial F(0 Complex Subunit B1 antibodyAnti-Atp Synthase Subunit B antibodyAnti-Atpase Subunit B antibodyAnti-ATP5PB antibodyAnti-ATP5F1 antibody |
Information sourced from Uniprot.org