• Immunoprecipitation analysis of 200 Mu g extracts of LO2 cells, using 3 Mu g ATP1A1 antibody (STJ22716). Western blot was performed from the immunoprecipitate using ATP1A1 antibody (STJ22716) at a dilution of 1:1000.
  • Immunohistochemistry analysis of ATP1A in paraffin-embedded human stomach using ATP1A Rabbit polyclonal antibody (STJ22716) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ATP1A in paraffin-embedded human prostate using ATP1A Rabbit polyclonal antibody (STJ22716) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using ATP1A Rabbit polyclonal antibody (STJ22716) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using ATP1A Rabbit polyclonal antibody (STJ22716) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ATP1A antibody (551-850) (STJ22716)

SKU:
STJ22716

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP1A (551-850) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP1A1
Gene ID: 476
Uniprot ID: AT1A1_HUMAN
Immunogen Region: 551-850
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human ATP1A (NP_000692.2).
Immunogen Sequence: RVLGFCHLFLPDEQFPEGFQ FDTDDVNFPIDNLCFVGLIS MIDPPRAAVPDAVGKCRSAG IKVIMVTGDHPITAKAIAKG VGIISEGNETVEDIAARLNI PVSQVNPRDAKACVVHGSDL KDMTSEQLDDILKYHTEIVF ARTSPQQKLIIVEGCQRQGA IVAVTGDGVNDSPALKKADI GVAMGIAGSDVSKQAADMIL LDDNFASIVTGVEEGRLIFD NLKKSIAYTLTSNIPEITP
Post Translational Modifications Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity.
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Protein Name Sodium/Potassium-Transporting Atpase Subunit Alpha-1
Na(+/K(+ Atpase Alpha-1 Subunit
Sodium Pump Subunit Alpha-1
Database Links Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Reactome: R-HSA-9679191
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Sarcolemma
Cell Projection
Axon
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Sodium/Potassium-Transporting Atpase Subunit Alpha-1 antibody
Anti-Na(+/K(+ Atpase Alpha-1 Subunit antibody
Anti-Sodium Pump Subunit Alpha-1 antibody
Anti-ATP1A1 antibody

Information sourced from Uniprot.org

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