| Host: |
Mouse |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-ATG3 is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
SM5121 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB (H, M, R) 1:500-1:1000 |
| Storage Instruction: |
Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
| Gene Symbol: |
Atg3 |
| Gene ID: |
67841 |
| Uniprot ID: |
ATG3_MOUSE |
| Immunogen: |
KLH conjugated Synthetic peptide corresponding to Mouse ATG3 |
| Function | E2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation. Cycles back and forth between binding to ATG7 for loading with the ATG8-like proteins and binding to E3 enzyme, composed of ATG12, ATG5 and ATG16L1 to promote ATG8-like proteins lipidation. Also play a role as a membrane curvature sensor that facilitates LC3/GABARAP lipidation by sensing local membrane stress associated with lipid-packing defects as occurs with high molar proportions of conical lipids or strident membrane curvature. Interacts with negatively-charged membranes promoting membrane tethering and enhancing LC3/GABARAP lipidation. Also acts as an autocatalytic E2-like enzyme by catalyzing the conjugation of ATG12 to itself in an ATG7-dependent manner, this complex thus formed, plays a role in mitochondrial homeostasis but not in autophagy. ATG12-ATG3 conjugation promotes late endosome to lysosome trafficking and basal autophagosome maturation via its interaction with PDCD6IP. ATG12-ATG3 conjugate is also formed upon viccina virus infection, leading to the disruption the cellular autophagy which is not necessary for vaccinia survival and proliferation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. |
| Protein Name | Ubiquitin-Like-Conjugating Enzyme Atg3Autophagy-Related Protein 3Apg3-Like |
| Database Links | Reactome: R-MMU-1632852 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin-Like-Conjugating Enzyme Atg3 antibodyAnti-Autophagy-Related Protein 3 antibodyAnti-Apg3-Like antibodyAnti-Atg3 antibodyAnti-Apg3l antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
