Anti-ATG16L1 antibody (STJ13100004)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from human ATG16L1 conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in mouse and rat.
General Information
| Short Description | Rabbit polyclonal anti-ATG16L1 for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. Use at a dilution of 1:100 to 1:2000. The optimal dilution should be determined by the end user. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20ยฐC for long term storage and refrigerated at 2-8ยฐC for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | ATG16L1 |
| Gene ID | 55054 |
| Uniprot ID | A16L1_HUMAN |
| Immunogen | A synthetic peptide from human ATG16L1 conjugated to blue carrier protein has been used as the antigen. The peptide is homologous in mouse and rat. |
| Specificity | Specific for ATG16L1. |
Additional Info
| Function | Plays an essential role in both canonical and non-canonical autophagy: interacts with ATG12-ATG5 to mediate the lipidation to ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP). Acts as a molecular hub, coordinating autophagy pathways via distinct domains that support either canonical or non-canonical signaling. During canonical autophagy, interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to ATG8 proteins, to produce a membrane-bound activated form of ATG8. Thereby, controls the elongation of the nascent autophagosomal membrane. As part of the ATG8 conjugation system with ATG5 and ATG12, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress. Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, probably by catalyzing conjugation of phosphatidylserine (PS) to ATG8. Non-canonical autophagy plays a key role in epithelial cells to limit lethal infection by influenza A (IAV) virus. Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production. Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response. Instead, promotes an autophagy-dependent antibacterial pathway together with NOD1 or NOD2. Plays a role in regulating morphology and function of Paneth cell. |
| Protein Name | Autophagy-Related Protein 16-1Apg16-Like 1 |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | CytoplasmPreautophagosomal Structure MembranePeripheral Membrane ProteinEndosome MembraneLysosome MembraneRecruited To Omegasomes Membranes By Wipi2Omegasomes Are Endoplasmic Reticulum Connected Strutures At The Origin Of Preautophagosomal StructuresLocalized To Preautophagosomal Structure (Pas) Where It Is Involved In The Membrane Targeting Of Atg5Also Localizes To Discrete Punctae Along The Ciliary AxonemeUpon Activation Of Non-Canonical AutophagyRecruited To Single-Membrane Endolysosomal CompartmentsUnder Starved ConditionsThe Atg12-Atg5-Atg16l1 Complex Is Translocated To Phagophores Driven By Rab33b |
| Alternative Antibody Names | Anti-Autophagy-Related Protein 16-1 antibodyAnti-Apg16-Like 1 antibodyAnti-ATG16L1 antibodyAnti-APG16L antibodyAnti-UNQ9393 antibodyAnti-PRO34307 antibody |
Information sourced from Uniprot.org