• Immunofluorescence analysis of U2OS cells using ATG12 Rabbit polyclonal antibody (STJ118058) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ATG12 Rabbit polyclonal antibody (STJ118058) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ATG12 Rabbit polyclonal antibody (STJ118058) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ATG12 antibody (STJ118058) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-ATG12 antibody (1-100) (STJ118058)

SKU:
STJ118058

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG12
Gene ID: 9140
Uniprot ID: ATG12_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG12 (NP_004698.3).
Immunogen Sequence: MAEEPQSVLQLPTSIAAGGE GLTDVSPETTTPEPPSSAAV SPGTEEPAGDTKKKIDILLK AVGDTPIMKTKKWAVERTRT IQGLIDFIKKFLKLVASEQL
Tissue Specificity Ubiquitous.
Post Translational Modifications Acetylated by EP300.
Function Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. As part of the ATG8 conjugation system with ATG5 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress. (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established.
Protein Name Ubiquitin-Like Protein Atg12
Autophagy-Related Protein 12
Apg12-Like
Database Links Reactome: R-HSA-1632852
Reactome: R-HSA-5205685
Reactome: R-HSA-8934903
Reactome: R-HSA-936440
Cellular Localisation Cytoplasm
Preautophagosomal Structure Membrane
Peripheral Membrane Protein
Tecpr1 Recruits The Atg12-Atg5 Conjugate To The Autolysosomal Membrane
Alternative Antibody Names Anti-Ubiquitin-Like Protein Atg12 antibody
Anti-Autophagy-Related Protein 12 antibody
Anti-Apg12-Like antibody
Anti-ATG12 antibody
Anti-APG12 antibody
Anti-APG12L antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance