Anti-ATF7IP antibody (525-725) (STJ116407)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ATF7IP (525-725) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATF7IP |
| Gene ID | 55729 |
| Uniprot ID | MCAF1_HUMAN |
| Immunogen Region | 525-725 |
| Immunogen Sequence | NEKDNKPEEEEQVIHEDDER PSEKNEFSRRKRSKSEDMDN VQSKRRRYMEEEYEAEFQVK ITAKGDINQKLQKVIQWLLE EKLCALQCAVFDKTLAELKT RVEKIECNKRHKTVLTELQA KIARLTKRFEAAKEDLKKRH EHPPNPPVSPGKTVNDVNSN NNMSYRNAGTVRQMLESKRN VSESAPPSFQTPVNTVSSTN L |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 525-725 of human ATF7IP (NP_060649.3). |
Additional Info
| Tissue Specificity | Detected at low levels in breast, lung and stomach.highly up-regulated in the corresponding cancerous tissues (at protein level). |
| Function | Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Required for HUSH-mediated heterochromatin formation and gene silencing. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Stabilizes SETDB1, is required to stimulate histone methyltransferase activity of SETDB1 and facilitates the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells. |
| Protein Name | Activating Transcription Factor 7-Interacting Protein 1Atf-Interacting ProteinAtf-IpAtf7-Interacting ProteinAtfa-Associated ModulatorHamMbd1-Containing Chromatin-Associated Factor 1P621 |
| Database Links | Reactome: R-HSA-3214841Reactome: R-HSA-9843940Reactome: R-HSA-9843970 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Activating Transcription Factor 7-Interacting Protein 1 antibodyAnti-Atf-Interacting Protein antibodyAnti-Atf-Ip antibodyAnti-Atf7-Interacting Protein antibodyAnti-Atfa-Associated Modulator antibodyAnti-Ham antibodyAnti-Mbd1-Containing Chromatin-Associated Factor 1 antibodyAnti-P621 antibodyAnti-ATF7IP antibodyAnti-MCAF antibodyAnti-MCAF1 antibody |
Information sourced from Uniprot.org