• EB012080 (1µg/ml) staining of HeLa (A) and MCF7 (B) cell lysate, and HeLa (C) and Jurkat (D) nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72994 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72994 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72994 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-ATF6 antibody (437-449) (STJ72994)

SKU:
STJ72994-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ATF6 (437-449) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-2µg/ml
IF-Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ATF6
Gene ID: 22926
Uniprot ID: ATF6A_HUMAN
Immunogen Region: 437-449
Accession Number: NP_031374.2
Immunogen Sequence: NSYRYDHSVSNDK
Post Translational Modifications During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) proteases. N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR). Ubiquitinated by RNF186 at Lys-152, which is required for pattern recognition receptor-induced unfolded protein response-associated outcomes.
Function Cyclic AMP-dependent transcription factor ATF-6 alpha: Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 alpha), which is embedded in the endoplasmic reticulum membrane. Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR). Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Transcription factor that initiates the unfolded protein response (UPR) during endoplasmic reticulum stress by activating transcription of genes involved in the UPR. Binds DNA on the 5'-CCACGA-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCACGA-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.
Protein Name Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha
Camp-Dependent Transcription Factor Atf-6 Alpha
Activating Transcription Factor 6 Alpha
Atf6-Alpha Cleaved Into - Processed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha
Database Links Reactome: R-HSA-380994
Reactome: R-HSA-381033
Reactome: R-HSA-381183
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Golgi Apparatus Membrane
Translocates From The Endoplasmic Reticulum To The Golgi
Where It Is Processed
Processed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha: Nucleus
Under Er Stress The Cleaved N-Terminal Cytoplasmic Domain Translocates Into The Nucleus
Thbs4 Promotes Its Nuclear Shuttling
Alternative Antibody Names Anti-Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha antibody
Anti-Camp-Dependent Transcription Factor Atf-6 Alpha antibody
Anti-Activating Transcription Factor 6 Alpha antibody
Anti-Atf6-Alpha Cleaved Into - Processed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha antibody
Anti-ATF6 antibody

Information sourced from Uniprot.org

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