• Western blot analysis of extracts of various cell lines, using ASS1 rabbit monoclonal antibody (STJ11102420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH-3T3 cells using ASS1 Rabbit monoclonal antibody (STJ11102420) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using ASS1 rabbit monoclonal antibody (STJ11102420) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ASS1 Rabbit monoclonal antibody (STJ11102420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ASS1 antibody (1-100) [S0MR] (STJ11102420)

SKU:
STJ11102420

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ASS1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASS1
Gene ID: 445
Uniprot ID: ASSY_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ASS1 (P00966).
Immunogen Sequence: MSSKGSVVLAYSGGLDTSCI LVWLKEQGYDVIAYLANIGQ KEDFEEARKKALKLGAKKVF IEDVSREFVEEFIWPAIQSS ALYEDRYLLGTSLARPCIAR
Tissue Specificity Expressed in adult liver.
Post Translational Modifications Acetylated by CLOCK in a circadian manner which negatively regulates its enzyme activity. Deacetylated by histone deacetylases.
Function One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Protein Name Argininosuccinate Synthase
Citrulline--Aspartate Ligase
Database Links Reactome: R-HSA-70635
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Argininosuccinate Synthase antibody
Anti-Citrulline--Aspartate Ligase antibody
Anti-ASS1 antibody
Anti-ASS antibody

Information sourced from Uniprot.org

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