Anti-ASRGL1/ALP antibody (Internal) (STJ71356)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ71356-100
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General Information

Short DescriptionGoat polyclonal antibody anti-ASRGL1/ALP (Internal) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 34kDa band observed in Human Tosil and lysteof cell line Hela lysates (calculated MW of 32.1kDa according to NP_001077395.1). Recommended concentration: 1-3µg/ml. An additional
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolASRGL1
Gene ID80150
Uniprot IDASGL1_HUMAN
Accession NumberNP_001077395.1; NP_079356.3
Immunogen RegionInternal
Immunogen SequenceEKHEKGAQKTDCQ
SpecificityBoth reported variants represent identical protein (NP_001077395.1 and NP_079356.3).

Additional Info

Post Translational Modifications Cleaved into an alpha and beta chain by autocatalysis.this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity.
Function Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.
Protein Name Isoaspartyl Peptidase/L-Asparaginase
Asparaginase-Like Protein 1
Beta-Aspartyl-Peptidase
Isoaspartyl Dipeptidase
L-Asparagine Amidohydrolase Cleaved Into - Isoaspartyl Peptidase/L-Asparaginase Alpha Chain - Isoaspartyl Peptidase/L-Asparaginase Beta Chain
Database Links Reactome: R-HSA-8964208
Cellular Localisation Cytoplasm
Midpiece Of Sperm Tail
Alternative Antibody Names Anti-Isoaspartyl Peptidase/L-Asparaginase antibody
Anti-Asparaginase-Like Protein 1 antibody
Anti-Beta-Aspartyl-Peptidase antibody
Anti-Isoaspartyl Dipeptidase antibody
Anti-L-Asparagine Amidohydrolase Cleaved Into - Isoaspartyl Peptidase/L-Asparaginase Alpha Chain - Isoaspartyl Peptidase/L-Asparaginase Beta Chain antibody
Anti-ASRGL1 antibody
Anti-ALP antibody
Anti-CRASH antibody

Information sourced from Uniprot.org

Citations

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