• Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells, using 3 Mu g TUG/ASPSCR1 antibody (STJ29617). Western blot was performed from the immunoprecipitate using TUG/ASPSCR1 antibody (STJ29617) at a dilution of 1:1000.
  • Immunofluorescence analysis of PC-12 cells using TUG/ASPSCR1 Rabbit polyclonal antibody (STJ29617) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using TUG/ASPSCR1 Rabbit polyclonal antibody (STJ29617) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using TUG/ASPSCR1 Rabbit polyclonal antibody (STJ29617) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using TUG/ASPSCR1 Rabbit polyclonal antibody (STJ29617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ASPSCR1 antibody (284-553) (STJ29617)

SKU:
STJ29617

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ASPSCR1 (284-553) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASPSCR1
Gene ID: 79058
Uniprot ID: ASPC1_HUMAN
Immunogen Region: 284-553
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human TUG/ASPSCR1 (NP_076988.1).
Immunogen Sequence: KSKSGQDPQQEQEQERERDP QQEQERERPVDREPVDREPV VCHPDLEERLQAWPAELPDE FFELTVDDVRRRLAQLKSER KRLEEAPLVTKAFREAQIKE KLERYPKVALRVLFPDRYVL QGFFRPSETVGDLRDFVRSH LGNPELSFYLFITPPKTVLD DHTQTLFQANLFPAALVHLG AEEPAGVYLEPGLLEHAISP SAADVLVARYMSRAAGSPSP LPAPDPAPKSEPAAEEGAL
Tissue Specificity Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.
Function Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface. Enhances VCP methylation catalyzed by VCPKMT.
Protein Name Tether Containing Ubx Domain For Glut4
Alveolar Soft Part Sarcoma Chromosomal Region Candidate Gene 1 Protein
Alveolar Soft Part Sarcoma Locus
Renal Papillary Cell Carcinoma Protein 17
Ubx Domain-Containing Protein 9
Database Links Reactome: R-HSA-1445148
Cellular Localisation Endomembrane System
Peripheral Membrane Protein
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Cytoplasm
Nucleus
Alternative Antibody Names Anti-Tether Containing Ubx Domain For Glut4 antibody
Anti-Alveolar Soft Part Sarcoma Chromosomal Region Candidate Gene 1 Protein antibody
Anti-Alveolar Soft Part Sarcoma Locus antibody
Anti-Renal Papillary Cell Carcinoma Protein 17 antibody
Anti-Ubx Domain-Containing Protein 9 antibody
Anti-ASPSCR1 antibody
Anti-ASPL antibody
Anti-RCC17 antibody
Anti-TUG antibody
Anti-UBXD9 antibody
Anti-UBXN9 antibody

Information sourced from Uniprot.org

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