• Immunofluorescence analysis of MCF-7 cells using ASPA antibody (STJ29410). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ASPA Antibody (STJ29410) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ASPA antibody (1-313) (STJ29410)

SKU:
STJ29410

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ASPA (1-313) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ASPA
Gene ID: 443
Uniprot ID: ACY2_HUMAN
Immunogen Region: 1-313
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human ASPA (NP_001121557.1).
Immunogen Sequence: MTSCHIAEEHIQKVAIFGGT HGNELTGVFLVKHWLENGAE IQRTGLEVKPFITNPRAVKK CTRYIDCDLNRIFDLENLGK KMSEDLPYEVRRAQEINHLF GPKDSEDSYDIIFDLHNTTS NMGCTLILEDSRNNFLIQMF HYIKTSLAPLPCYVYLIEHP SLKYATTRSIAKYPVGIEVG PQPQGVLRADILDQMRKMIK HALDFIHHFNEGKEFPPCAI EVYKIIEKVDYPRDENGEI
Tissue Specificity Brain white matter, skeletal muscle, kidney, adrenal glands, lung and liver.
Function Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it acts as a scavenger of NAA from body fluids.
Protein Name Aspartoacylase
Aminoacylase-2
Acy-2
Database Links Reactome: R-HSA-8963693
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Aspartoacylase antibody
Anti-Aminoacylase-2 antibody
Anti-Acy-2 antibody
Anti-ASPA antibody
Anti-ACY2 antibody
Anti-ASP antibody

Information sourced from Uniprot.org

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