Anti-ASL antibody (289-464) (STJ28440)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ASL (289-464) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ASL |
| Gene ID | 435 |
| Uniprot ID | ARLY_HUMAN |
| Immunogen Region | 289-464 |
| Immunogen Sequence | NPDSLELIRSKAGRVFGRCA GLLMTLKGLPSTYNKDLQED KEAVFEVSDTMSAVLQVATG VISTLQIHQENMGQALSPDM LATDLAYYLVRKGMPFRQAH EASGKAVFMAETKGVALNQL SLQELQTISPLFSGDVICVW DYGHSVEQYGALGGTARSSV DWQIRQVRALLQAQQA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 289-464 of human ASL (NP_000039.2). |
Additional Info
| Post Translational Modifications | Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity. |
| Function | Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues. Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway. |
| Protein Name | Argininosuccinate LyaseAsalArginosuccinase |
| Database Links | Reactome: R-HSA-70635 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Argininosuccinate Lyase antibodyAnti-Asal antibodyAnti-Arginosuccinase antibodyAnti-ASL antibody |
Information sourced from Uniprot.org