• Western blot analysis of the lysates from HepG2 cells using ARSI antibody.
  • Western blot analysis of lysates from COS7 cells, using ARSI Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of MCF7 cells, using ARSI Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Arylsulfatase I Polyclonal Antibody

Anti-ARSI antibody (311-360 aa) (STJ91717)

SKU:
STJ91717

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Arylsulfatase I (311-360 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARSI
Gene ID: 340075
Uniprot ID: ARSI_HUMAN
Immunogen Region: 311-360 aa
Specificity: Arylsulfatase I Polyclonal Antibody detects endogenous levels of Arylsulfatase I protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ARSI at the amino acid range 311-360
Post Translational Modifications The oxidation of Cys-93 residue to 3-oxoalanine (also known as C(alpha)-formylglycine) by SUMF1/Sulfatase-modifying factor 1, seems critical for catalytic activity.
Function Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1.arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured in cell extracts. Lacks arylsulfatase activity.
Protein Name Arylsulfatase I
Asi
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-1663150
Cellular Localisation Secreted
Endoplasmic Reticulum
Localized In The Intracellular Granular Structures
Alternative Antibody Names Anti-Arylsulfatase I antibody
Anti-Asi antibody
Anti-ARSI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance