Anti-ARSG antibody (251-300 aa) (STJ91716)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human ARSG at the amino acid range 251-300
General Information
| Short Description | Rabbit polyclonal anti-Arylsulfatase G (251-300 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARSG |
| Gene ID | 22901 |
| Uniprot ID | ARSG_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human ARSG at the amino acid range 251-300 |
| Immunogen Region | 251-300 aa |
| Specificity | Arylsulfatase G Polyclonal Antibody detects endogenous levels of Arylsulfatase G protein. |
Additional Info
| Post Translational Modifications | N-glycosylated. N-glycosylated with both high mannose and complex type sugars. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. The 63-kDa precursor undergoes proteolytic processing in two steps, yielding two fragments in the first step (apparent molecular masses of 44 and 18 kDa). In the second step, the 44-kDa fragment is processed further to the 34- and 10-kDa chains. The 10-kDa chain is a cleavage product of the 44-kDa fragment but linked to the 18-kDa chain through a disulfide bridge. |
| Function | Displays arylsulfatase activity at acidic pH towards artificial substrates, such as p-nitrocatechol sulfate and also, but with a lower activity towards p-nitrophenyl sulfate and 4-methylumbelliferyl sulfate. Catalyzes the hydrolysis of the 3-sulfate groups of the N-sulfo-D-glucosamine 3-O-sulfate units of heparin. |
| Protein Name | Arylsulfatase GAsgN-Sulfoglucosamine-3-Sulfatase |
| Database Links | Reactome: R-HSA-1663150Reactome: R-HSA-9840310 |
| Cellular Localisation | LysosomeThe 63-Kda Precursor Protein Localizes To Pre-Lysosomal Compartments And Tightly Associates With Organelle MembranesMost Likely The Endoplasmic ReticulumIn ContrastProteolytically Processed Fragments Of 34-18- And 10-Kda Are Found In Lysosomal Fractions And Lose Their Membrane Association |
| Alternative Antibody Names | Anti-Arylsulfatase G antibodyAnti-Asg antibodyAnti-N-Sulfoglucosamine-3-Sulfatase antibodyAnti-ARSG antibodyAnti-KIAA1001 antibodyAnti-UNQ839 antibodyAnti-PRO1777 antibody |
Information sourced from Uniprot.org