Anti-ARIH1/HHARI antibody (C-Term) (STJ70308)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ARIH1/HHARI (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1ยตg/ml. Primary incubated for 1 hour.IHC: Paraffin embedde |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ARIH1 |
| Gene ID | 25820 |
| Uniprot ID | ARI1_HUMAN |
| Accession Number | NP_005735.2 |
| Immunogen Region | C-Term |
| Immunogen Sequence | HEGYEKDLWEYIED |
Additional Info
| Function | E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets. The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes. Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear. According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report EIF4E2 ubiquitination leads to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2. In vitro, controls the degradation of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex member SUN2 and may therefore have a role in the formation and localization of the LINC complex, and as a consequence, nuclear subcellular localization and nuclear morphology. |
| Protein Name | E3 Ubiquitin-Protein Ligase Arih1H7-Ap2HhariMonocyte Protein 6Mop-6Protein Ariadne-1 HomologAri-1Ubch7-Binding ProteinUbcm4-Interacting ProteinUbiquitin-Conjugating Enzyme E2-Binding Protein 1 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-9833110Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmNucleusCajal BodyMainly CytoplasmicPresent In Lewy Body |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Arih1 antibodyAnti-H7-Ap2 antibodyAnti-Hhari antibodyAnti-Monocyte Protein 6 antibodyAnti-Mop-6 antibodyAnti-Protein Ariadne-1 Homolog antibodyAnti-Ari-1 antibodyAnti-Ubch7-Binding Protein antibodyAnti-Ubcm4-Interacting Protein antibodyAnti-Ubiquitin-Conjugating Enzyme E2-Binding Protein 1 antibodyAnti-ARIH1 antibodyAnti-ARI antibodyAnti-MOP6 antibodyAnti-UBCH7BP antibodyAnti-HUSSY-27 antibody |
Information sourced from Uniprot.org