• Immunoprecipitation analysis of 150 Mu g extracts of MCF7 cells using 3 Mu g ARID3A antibody (STJ29981). Western blot was performed from the immunoprecipitate using ARID3A antibody (STJ29981) at a dilution of 1:500.
  • Immunohistochemistry analysis of ARID3A in paraffin-embedded human lung cancer using ARID3A Rabbit polyclonal antibody (STJ29981) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ARID3A in paraffin-embedded rat lung using ARID3A Rabbit polyclonal antibody (STJ29981) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from K-562 cells using ARID3A Rabbit polyclonal antibody (STJ29981) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:10s.

Anti-ARID3A antibody (324-593) (STJ29981)

SKU:
STJ29981

€211.88 - €561.88
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARID3A (324-593) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARID3A
Gene ID: 1820
Uniprot ID: ARI3A_HUMAN
Immunogen Region: 324-593
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 324-593 of human ARID3A (NP_005215.1).
Immunogen Sequence: PYECEKRGLSNPNELQAAID SNRREGRRQSFGGSLFAYSP GGAHGMLSSPKLPVSSLGLA ASTNGSSITPAPKIKKEEDS AIPITVPGRLPVSLAGHPVV AAQAAAVQAAAAQAAVAAQA AALEQLREKLESAEPPEKKM ALVADEQQRLMQRALQQNFL AMAAQLPMSIRINSQASESR QDSAVNLTGTNGSNSISMSV EINGIMYTGVLFAQPPAPTP TSAPNKGGGGGGGSSSNAG
Tissue Specificity Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.
Function Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
Protein Name At-Rich Interactive Domain-Containing Protein 3a
Arid Domain-Containing Protein 3a
B-Cell Regulator Of Igh Transcription
Bright
Dead Ringer-Like Protein 1
E2f-Binding Protein 1
Database Links Reactome: R-HSA-6804116
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between Nucleus And Cytoplasm
Alternative Antibody Names Anti-At-Rich Interactive Domain-Containing Protein 3a antibody
Anti-Arid Domain-Containing Protein 3a antibody
Anti-B-Cell Regulator Of Igh Transcription antibody
Anti-Bright antibody
Anti-Dead Ringer-Like Protein 1 antibody
Anti-E2f-Binding Protein 1 antibody
Anti-ARID3A antibody
Anti-DRIL1 antibody
Anti-DRIL3 antibody
Anti-DRX antibody
Anti-E2FBP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance