• Western blot analysis of extracts of LO2 cells, using ARID2 antibody (STJ111333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293F cells using 3 Mu g ARID2 Rabbit polyclonal antibody (STJ111333). Western blot was performed from the immunoprecipitate using ARID2 Rabbit polyclonal antibody (STJ111333) at a dilition of 1:1000.
  • Western blot analysis of extracts from normal (control) and ARID2 knockout (KO) HeLa cells, using ARID2 antibody (STJ111333) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from LO2 cells, using ARID2 Rabbit polyclonal antibody (STJ111333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ARID2 antibody (1586-1835) (STJ111333)

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STJ111333

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARID2 (1586-1835) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARID2
Gene ID: 196528
Uniprot ID: ARID2_HUMAN
Immunogen Region: 1586-1835
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1586-1835 of human ARID2 (NP_689854.2).
Immunogen Sequence: QNVVPQNTPMPPSPAVQVQG QPNSSQPSPFSGSSQPGDPM RKPGQNFMCLWQSCKKWFQT PSQVFYHAATEHGGKDVYPG QCLWEGCEPFQRQRFSFITH LQDKHCSKDALLAGLKQDEP GQAGSQKSSTKQPTVGGTSS TPRAQKAIVNHPSAALMALR RGSRNLVFRDFTDEKEGPIT KHIRLTAALILKNIGKYSEC GRRLLKRHENNLSVLAISNM EASSTLAKCLYELNFTVQS
Tissue Specificity Highly expressed in heart.
Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). May be involved in targeting the complex to different genes. May be involved in regulating transcriptional activation of cardiac genes.
Protein Name At-Rich Interactive Domain-Containing Protein 2
Arid Domain-Containing Protein 2
Brg1-Associated Factor 200
Baf200
Zinc Finger Protein With Activation Potential
Zipzap/P200
Database Links Reactome: R-HSA-3214858
Reactome: R-HSA-8939243
Cellular Localisation Nucleus
Alternative Antibody Names Anti-At-Rich Interactive Domain-Containing Protein 2 antibody
Anti-Arid Domain-Containing Protein 2 antibody
Anti-Brg1-Associated Factor 200 antibody
Anti-Baf200 antibody
Anti-Zinc Finger Protein With Activation Potential antibody
Anti-Zipzap/P200 antibody
Anti-ARID2 antibody
Anti-BAF200 antibody
Anti-KIAA1557 antibody

Information sourced from Uniprot.org

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