• Western blot analysis of extracts of various cell lines, using ARHGEF9 antibody (STJ110271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using ARHGEF9 antibody (STJ110271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARHGEF9 antibody (307-516) (STJ110271)

SKU:
STJ110271

€140.63 - €655.63
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGEF9
Gene ID: 23229
Uniprot ID: ARHG9_HUMAN
Immunogen Region: 307-516
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 307-516 of human ARHGEF9 (NP_056000.1).
Immunogen Sequence: WEGEDILDRSSELIYTGEMA WIYQPYGRNQQRVFFLFDHQ MVLCKKDLIRRDILYYKGRI DMDKYEVVDIEDGRDDDFNV SMKNAFKLHNKETEEIHLFF AKKLEEKIRWLRAFREERKM VQEDEKIGFEISENQKRQAA MTVRKVPKQKGVNSARSVPP SYPPPQDPLNHGQYLVPDGI AQSQVFEFTEPKRSQSPFWQ NFSRLTPFKK
Tissue Specificity Detected in brain. Detected at low levels in heart.
Function Acts as a guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters.
Protein Name Rho Guanine Nucleotide Exchange Factor 9
Collybistin
Pem-2 Homolog
Rac/Cdc42 Guanine Nucleotide Exchange Factor 9
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-416482
Reactome: R-HSA-9013148
Reactome: R-HSA-9013406
Reactome: R-HSA-977443
Cellular Localisation Cytoplasm
Postsynaptic Density
Alternative Antibody Names Anti-Rho Guanine Nucleotide Exchange Factor 9 antibody
Anti-Collybistin antibody
Anti-Pem-2 Homolog antibody
Anti-Rac/Cdc42 Guanine Nucleotide Exchange Factor 9 antibody
Anti-ARHGEF9 antibody
Anti-ARHDH9 antibody
Anti-KIAA0424 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance