| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-ARHGEF4 (N-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-2-4µg/mlELISA-antibody detection limit dilution 1:32000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
ARHGEF4 |
| Gene ID: |
50649 |
| Uniprot ID: |
ARHG4_HUMAN |
| Immunogen Region: |
N-Term |
| Accession Number: |
NP_056135.2; NP_127462.1 |
| Specificity: |
This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1). |
| Immunogen Sequence: |
PWEEPAGEKPSCS |
| Function | Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 4Apc-Stimulated Guanine Nucleotide Exchange Factor 1AsefAsef1 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | Isoform 3: CytoplasmCell ProjectionRuffle MembranePeripheral Membrane ProteinCytoplasmic SideAssociated With Membrane Ruffles |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 4 antibodyAnti-Apc-Stimulated Guanine Nucleotide Exchange Factor 1 antibodyAnti-Asef antibodyAnti-Asef1 antibodyAnti-ARHGEF4 antibodyAnti-KIAA1112 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
