• Western blot analysis of extracts of mouse brain, using ARHGEF11 antibody (STJ110263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using ARHGEF11 antibody (STJ110263) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human prostate cancer using ARHGEF11 antibody (STJ110263) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human lung using ARHGEF11 antibody (STJ110263) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human lung using ARHGEF11 antibody (STJ110263) (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human prostate cancer using ARHGEF11 antibody (STJ110263) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using ARHGEF11 antibody (STJ110263) (40x lens).
  • Western blot analysis of Mouse lung, using ARHGEF11 Rabbit polyclonal antibody (STJ110263) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ARHGEF11 antibody (1-100) (STJ110263)

SKU:
STJ110263

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARHGEF11 (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGEF11
Gene ID: 9826
Uniprot ID: ARHGB_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ARHGEF11 (O15085).
Immunogen Sequence: MSVRLPQSIDRLSSLSSLGD SAPERKSPSHHRQPSDASET TGLVQRCVIIQKDQHGFGFT VSGDRIVLVQSVRPGGAAMK AGVKEGDRIIKVNGTMVTNS
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14). Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex when previously phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14), leading to its degradation, thereby restricting RhoA activity and facilitating growth cone spreading and neurite outgrowth.
Function May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Involved in neurotrophin-induced neurite outgrowth.
Protein Name Rho Guanine Nucleotide Exchange Factor 11
Pdz-Rhogef
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-416482
Reactome: R-HSA-416572
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Cellular Localisation Cytoplasm
Membrane
Translocated To The Membrane Upon Stimulation
Alternative Antibody Names Anti-Rho Guanine Nucleotide Exchange Factor 11 antibody
Anti-Pdz-Rhogef antibody
Anti-ARHGEF11 antibody
Anti-KIAA0380 antibody

Information sourced from Uniprot.org

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