![Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95232/377118/STJ116239_1__44864.1713144353.jpg?c=1 "Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and ARHGEF1 knockout (KO) 293T cells, using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95232/377119/STJ116239_2__74076.1713144354.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ARHGEF1 knockout (KO) 293T cells, using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of various lysates using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95232/377120/STJ116239_3__99060.1713144354.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/95232/377118/STJ116239_1__44864.1713144353.jpg?c=1 "Immunohistochemistry analysis of ARHGEF1 in paraffin-embedded human tonsil using [KO Validated] ARHGEF1 Rabbit polyclonal antibody (STJ116239) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
Anti-ARHGEF1 antibody (783-912) (STJ116239)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ARHGEF1 (783-912) antibody for use in WB, IHC-P and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGEF1 |
| Gene ID | 9138 |
| Uniprot ID | ARHG1_HUMAN |
| Immunogen Region | 783-912 |
| Immunogen Sequence | PLLSSSENGNGGRETSPADA RTERILSDLLPFCRPGPEGQ LAATALRKVLSLKQLLFPAE EDNGAGPPRDGDGVPGGGPL SPARTQEIQENLLSLEETMK QLEELEEEFCRLRPLLSQLG GNSVPQPGCT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2. |
| Function | Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. In lymphoid follicles, may trigger activation of GNA13 as part of S1PR2-dependent signaling pathway that leads to inhibition of germinal center (GC) B cell growth and migration outside the GC niche. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 1115 Kda Guanine Nucleotide Exchange FactorP115-RhogefP115rhogefSub1.5 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106 |
| Cellular Localisation | CytoplasmMembraneTranslocated To The Membrane By Activated Gna13 Or Lpa Stimulation |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 1 antibodyAnti-115 Kda Guanine Nucleotide Exchange Factor antibodyAnti-P115-Rhogef antibodyAnti-P115rhogef antibodyAnti-Sub1.5 antibodyAnti-ARHGEF1 antibody |
Information sourced from Uniprot.org