• Immunofluorescence analysis of L929 cells using ARHGAP4 Rabbit polyclonal antibody (STJ11100294) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ARHGAP4 in paraffin-embedded mouse heart using ARHGAP4 Rabbit polyclonal antibody (STJ11100294) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ARHGAP4 in paraffin-embedded human thyroid cancer using ARHGAP4 Rabbit polyclonal antibody (STJ11100294) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ARHGAP4 in paraffin-embedded rat heart using ARHGAP4 Rabbit polyclonal antibody (STJ11100294) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Jurkat cells, using ARHGAP4 Rabbit polyclonal antibody (STJ11100294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARHGAP4 antibody (STJ11100294)

SKU:
STJ11100294

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARHGAP4 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGAP4
Gene ID: 393
Uniprot ID: RHG04_HUMAN
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 900 to the C-terminus of human ARHGAP4 (NP_001158213.1).
Immunogen Sequence: SPEQHVEVDKAVAQNMDSVF KELLGKTSVRQGLGPASTTS PSPGPRSPKAPPSSRLGRNK GFSRGPGAPASPSASHPQGL DTTPKPH
Tissue Specificity Predominantly in hematopoietic cells (spleen, thymus and leukocytes).low levels in placenta, lung and various fetal tissues.
Function Inhibitory effect on stress fiber organization. May down-regulate Rho-like GTPase in hematopoietic cells.
Protein Name Rho Gtpase-Activating Protein 4
Rho-Gap Hematopoietic Protein C1
Rho-Type Gtpase-Activating Protein 4
P115
Database Links Reactome: R-HSA-8980692
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Cellular Localisation Cytoplasm
Just Below The Plasma Membrane
Alternative Antibody Names Anti-Rho Gtpase-Activating Protein 4 antibody
Anti-Rho-Gap Hematopoietic Protein C1 antibody
Anti-Rho-Type Gtpase-Activating Protein 4 antibody
Anti-P115 antibody
Anti-ARHGAP4 antibody
Anti-KIAA0131 antibody
Anti-RGC1 antibody
Anti-RHOGAP4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance