• Immunofluorescence analysis of L929 cells using ARHGAP25 Polyclonal Antibody (STJ22673) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using ARHGAP25 Polyclonal Antibody (STJ22673) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded Human gastric using ARHGAP25 antibody (STJ22673) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using ARHGAP25 antibody (STJ22673) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ARHGAP25 antibody (STJ22673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ARHGAP25 antibody (1-370) (STJ22673)

SKU:
STJ22673

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARHGAP25 (1-370) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARHGAP25
Gene ID: 9938
Uniprot ID: RHG25_HUMAN
Immunogen Region: 1-370
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human ARHGAP25 (NP_001159748.1).
Immunogen Sequence: MSLGQSACLFLSIARSRSVM TGEQMAAFHPSSTPNPLERP IKMGWLKKQRSIVKNWQQRY FVLRAQQLYYYKDEEDTKPQ GCMYLPGCTIKEIATNPEEA GKFVFEIIPASWDQNRMGQD SYVLMASSQAEMEEWVKFLR RVAGTPCGAVFGQRLDETVA YEQKFGPHLVPILVEKCAEF ILEHGRNEEGIFRLPGQDNL VKQLRDAFDAGERPSFDRDT DVHTVASLLKLYLRDLPEP
Function GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
Protein Name Rho Gtpase-Activating Protein 25
Rho-Type Gtpase-Activating Protein 25
Database Links Reactome: R-HSA-9013149
Alternative Antibody Names Anti-Rho Gtpase-Activating Protein 25 antibody
Anti-Rho-Type Gtpase-Activating Protein 25 antibody
Anti-ARHGAP25 antibody
Anti-KIAA0053 antibody

Information sourced from Uniprot.org

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