| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ARF6 |
| Gene ID: |
382 |
| Uniprot ID: |
ARF6_HUMAN |
| Immunogen Region: |
1-269 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 76-175 of human ARF6 (NP_001654.1). |
| Immunogen Sequence: |
HYYTGTQGLIFVVDCADRDR IDEARQELHRIINDREMRDA IILIFANKQDLPDAMKPHEI QEKLGLTRIRDRNWYVQPSC ATSGDGLYEGLTWLTSNYKS |
| Tissue Specificity | Ubiquitous, with higher levels in heart, substantia nigra, and kidney. |
| Post Translational Modifications | GTP-bound form is myristoylated on Lys-3 by NMT1 and NMT2, allowing ARF6 to remain on membranes during the GTPase cycle, thereby promoting its activity. GDP-bound inactive form is demyristoylated on Lys-3 by SIRT2 at early endosomes or endocytic recycling compartment to allow its efficient activation by a guanine exchange factor (GEF) after GDP release. |
| Function | GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. GTP-bound form plays an important role in the transport of multiple palmitoylated proteins form the Golgi to the plasma membrane. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Potentiates the neurite outgrowth in primary neurons by interacting with the molecular adapter APBB1. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1. Required for NTRK1 sorting to the recycling pathway from early endosomes. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. (Microbial infection) Plays a key role in the endocytosis of enterovirus 71 and thus viral entry into brain microvascular endothelial cells. |
| Protein Name | Adp-Ribosylation Factor 6 |
| Database Links | Reactome: R-HSA-8854214Reactome: R-HSA-8856828Reactome: R-HSA-8875656 |
| Cellular Localisation | CytoplasmCytosolCell MembraneLipid-AnchorEndosome MembraneRecycling Endosome MembraneCell ProjectionFilopodium MembraneRuffleCleavage FurrowMidbodyMidbody RingEarly Endosome MembraneGolgi ApparatusTrans-Golgi Network MembraneDistributed Uniformly On The Plasma MembraneAs Well As Throughout The Cytoplasm During MetaphaseSubsequently Concentrated At Patches In The Equatorial Region At The Onset Of CytokinesisAnd Becomes Distributed In The Equatorial Region Concurrent With Cleavage Furrow IngressionIn Late Stages Of CytokinesisConcentrates At The Midbody Ring/Flemming BodyRecruitment To The Midbody Ring Requires Both Activation By Psd/Efa6a And Interaction With Kif23/Mklp1After Abscission Of The Intercellular BridgeIncorporated Into One Of The Daughter Cells As A Midbody Remnant And Localizes To Punctate Structures Beneath The Plasma MembraneRecruited To The Cell Membrane In Association With Cyth2 And Arl4cColocalizes With Dab2ip At The Plasma Membrane And Endocytic VesiclesMyristoylation Is Required For Proper Localization To Membranes: Myristoylation On Lys-3 Allows Arf6 To Remain On Membranes During The Gtpase Cycle |
| Alternative Antibody Names | Anti-Adp-Ribosylation Factor 6 antibodyAnti-ARF6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
