• Western blot analysis of lysates from wild type (WT) and ARF5 knockout (KO) 293T cells, using [KO Validated] ARF5 Rabbit polyclonal antibody (STJ11100862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ARF5 antibody (1-180) (STJ11100862)

SKU:
STJ11100862

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARF5 (1-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARF5
Gene ID: 381
Uniprot ID: ARF5_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ARF5 (NP_001653.1).
Immunogen Sequence: MGLTVSALFSRIFGKKQMRI LMVGLDAAGKTTILYKLKLG EIVTTIPTIGFNVETVEYKN ICFTVWDVGGQDKIRPLWRH YFQNTQGLIFVVDSNDRERV QESADELQKMLQEDELRDAV LLVFANKQDMPNAMPVSELT DKLGLQHLRSRTWYVQATCA TQGTGLYDGLDWLSHELSKR
Function GTP-binding protein involved in protein trafficking.may modulate vesicle budding and uncoating within the Golgi apparatus. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Protein Name Adp-Ribosylation Factor 5
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Golgi Apparatus
Cytoplasm
Perinuclear Region
Membrane
Lipid-Anchor
Trans-Golgi Network Membrane
Alternative Antibody Names Anti-Adp-Ribosylation Factor 5 antibody
Anti-ARF5 antibody

Information sourced from Uniprot.org

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