• Immunohistochemistry with Anti-ARF1 Antibody (STJ500153) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-ARF1 antibody (STJ500153)

SKU:
STJ500153-100

£481.50
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Host: Rabbit
Applications: ELISA/ICC/IF/IP/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARF1 is suitable for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.58-0.65 µg/µl
Dilution Range: WB: 1:500
DB: 1:10, 000
ELISA: 1:10, 000
IP: 1:100
ICC: Use at an assay dependent concentration.
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: Arf1
Gene ID: 64310
Uniprot ID: ARF1_RAT
Specificity: Does not cross react to other members of the Ras family or to other ARF members. This antibody will label ARF isoform 1, 2, 3, and 4.
Immunogen: Synthetic peptide corresponding to unique amino acid sequence on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desied antigenicity.
Function Small GTPase involved in protein trafficking between different compartments. Modulates vesicle budding and uncoating within the Golgi complex. In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity.the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD).
Protein Name Adp-Ribosylation Factor 1
Database Links Reactome: R-RNO-1660499
Reactome: -RNO-1660514
Reactome: -RNO-199992
Reactome: -RNO-2132295
Reactome: -RNO-432720
Reactome: -RNO-432722
Reactome: -RNO-6807878
Reactome: -RNO-6811434
Reactome: -RNO-6811438
Cellular Localisation Golgi Apparatus Membrane
Lipid-Anchor
Cytoplasmic Side
Synapse
Synaptosome
Postsynaptic Density
In The Gdp-Bound Form
Associates Transiently With The Membranes Via Its Myristoylated N-Terminus Where Guanine Nucleotide-Exchange Factor (Gef)-Mediated Nucleotide Exchange Occurs
Following Nucleotide Exchange
The Gtp-Bound Form Undergoes A Conformational Change
Leading To The Exposure Of A Myristoylated N-Terminal Amphipathic Helix That Provides Stable Membrane Anchorage
Alternative Antibody Names Anti-Adp-Ribosylation Factor 1 antibody
Anti-Arf1 antibody

Information sourced from Uniprot.org

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