| Host: | Rabbit |
| Applications: | ELISA/ICC/IF/IP/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-ARF1 is suitable for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | FITC |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.58-0.65 µg/µl |
| Dilution Range: | WB: 1:500DB: 1:10, 000ELISA: 1:10, 000IP: 1:200ICC: Use at an assay dependent concentration |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | Arf1 |
| Gene ID: | 64310 |
| Uniprot ID: | ARF1_RAT |
| Immunogen: | Synthetic peptide corresponding to unique amino acid sequence on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desied antigenicity. |
| Function | Small GTPase involved in protein trafficking between different compartments. Modulates vesicle budding and uncoating within the Golgi complex. In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity.the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis. Plays also a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation. |
| Protein Name | Adp-Ribosylation Factor 1 |
| Database Links | Reactome: R-RNO-1660499Reactome: -RNO-1660514Reactome: -RNO-199992Reactome: -RNO-2132295Reactome: -RNO-432720Reactome: -RNO-432722Reactome: -RNO-6807878Reactome: -RNO-6811434Reactome: -RNO-6811438Reactome: -RNO-9845576 |
| Cellular Localisation | Golgi Apparatus MembraneLipid-AnchorCytoplasmic SideSynapseSynaptosomePostsynaptic DensityIn The Gdp-Bound FormAssociates Transiently With The Membranes Via Its Myristoylated N-Terminus Where Guanine Nucleotide-Exchange Factor (Gef)-Mediated Nucleotide Exchange OccursFollowing Nucleotide ExchangeThe Gtp-Bound Form Undergoes A Conformational ChangeLeading To The Exposure Of A Myristoylated N-Terminal Amphipathic Helix That Provides Stable Membrane Anchorage |
| Alternative Antibody Names | Anti-Adp-Ribosylation Factor 1 antibodyAnti-Arf1 antibody |
Information sourced from Uniprot.org
