| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-AQR (400-700) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AQR |
| Gene ID: |
9716 |
| Uniprot ID: |
AQR_HUMAN |
| Immunogen Region: |
400-700 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human AQR (NP_055506.1). |
| Immunogen Sequence: |
KEFLLELLVSRHERRISQIQ QLNQMPLYPTEKIIWDENIV PTEYYSGEGCLALPKLNLQF LTLHDYLLRNFNLFRLESTY EIRQDIEDSVSRMKPWQSEY GGVVFGGWARMAQPIVAFTV VEVAKPNIGENWPTRVRADV TINLNVRDHIKDEWEGLRKH DVCFLITVRPTKPYGTKFDR RRPFIEQVGLVYVRGCEIQG MLDDKGRVIEDGPEPRPNLR GESRTFRVFLDPNQYQQDM |
| Function | Involved in pre-mRNA splicing as component of the spliceosome. Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing. Has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang (in vitro). |
| Protein Name | Rna Helicase AquariusIntron-Binding Protein Of 160 KdaIbp160 |
| Database Links | Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-72163 |
| Cellular Localisation | NucleusNucleoplasmLocalizes To Speckle-Like Regions Of The Nucleoplasm |
| Alternative Antibody Names | Anti-Rna Helicase Aquarius antibodyAnti-Intron-Binding Protein Of 160 Kda antibodyAnti-Ibp160 antibodyAnti-AQR antibodyAnti-KIAA0560 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89094/357553/STJ11100022_1__83889.1713121675.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and HERC4 knockout (KO) HeLa cells, using [KO Validated] HERC4 Rabbit polyclonal antibody (STJ11100022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")