• Western blot analysis of extracts of various cell lines, using AQP5 antibody (STJ111968) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using AQP5 Rabbit polyclonal antibody (STJ111968) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using AQP5 antibody (STJ111968) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using AQP5 Rabbit polyclonal antibody (STJ111968) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using AQP5 antibody (STJ111968) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using AQP5 Rabbit polyclonal antibody (STJ111968) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-AQP5 antibody (188-203) (STJ111968)

SKU:
STJ111968

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AQP5
Gene ID: 362
Uniprot ID: AQP5_HUMAN
Immunogen Region: 188-203
Specificity: A synthetic peptide corresponding to a sequence within amino acids 166-265 of human AQP5 (NP_001642.1).
Immunogen Sequence: GLSVTLGHLVGIYFTGCSMN PARSFGPAVVMNRFSPAHWV FWVGPIVGAVLAAILYFYLL FPNSLSLSERVAIIKGTYEP DEDWEEQREERKKTMELTTR
Tissue Specificity Detected in skin eccrine sweat glands, at the apical cell membrane and at intercellular canaliculi (at protein level).
Function Aquaporins form homotetrameric transmembrane channels, with each monomer independently mediating water transport across the plasma membrane along its osmotic gradient. Plays an important role in fluid secretion in salivary glands. Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells. Seems to play a redundant role in water transport in the eye, lung and in sweat glands.
Protein Name Aquaporin-5
Aqp-5
Database Links Reactome: R-HSA-432047
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Cytoplasmic Vesicle Membrane
Hypotonicity Increases Location At The Cell Membrane
Phosphorylation Decreases Location At The Cell Membrane
Alternative Antibody Names Anti-Aquaporin-5 antibody
Anti-Aqp-5 antibody
Anti-AQP5 antibody

Information sourced from Uniprot.org

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