• Western blot analysis of various cells using AQP2 Polyclonal Antibody
  • Immunofluorescence analysis of HeLa cells, using Aquaporin 2 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of Hela cell. 1, AQP2 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-AQP2 antibody (222-271 aa) (STJ91655)

SKU:
STJ91655

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Aquaporin-2 (222-271 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AQP2
Gene ID: 359
Uniprot ID: AQP2_HUMAN
Immunogen Region: 222-271 aa
Specificity: AQP2 Polyclonal Antibody detects endogenous levels of AQP2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Aquaporin 2 at the amino acid range 222-271
Post Translational Modifications Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. N-glycosylated.
Function Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis.
Protein Name Aquaporin-2
Aqp-2
Adh Water Channel
Aquaporin-Cd
Aqp-Cd
Collecting Duct Water Channel Protein
Wch-Cd
Water Channel Protein For Renal Collecting Duct
Database Links Reactome: R-HSA-432040
Reactome: R-HSA-432047
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Cell Membrane
Cytoplasmic Vesicle Membrane
Golgi Apparatus
Trans-Golgi Network Membrane
Shuttles From Vesicles To The Apical Membrane
Vasopressin-Regulated Phosphorylation Is Required For Translocation To The Apical Cell Membrane
Plekha8/Fapp2 Is Required To Transport Aqp2 From The Tgn To Sites Where Aqp2 Is Phosphorylated
Alternative Antibody Names Anti-Aquaporin-2 antibody
Anti-Aqp-2 antibody
Anti-Adh Water Channel antibody
Anti-Aquaporin-Cd antibody
Anti-Aqp-Cd antibody
Anti-Collecting Duct Water Channel Protein antibody
Anti-Wch-Cd antibody
Anti-Water Channel Protein For Renal Collecting Duct antibody
Anti-AQP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance