Anti-APTX antibody (93-342) (STJ27317)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal APTX (93-342) antibody for use in WB, IF, ICC, IP and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | APTX |
| Gene ID | 54840 |
| Uniprot ID | APTX_HUMAN |
| Immunogen Region | 93-342 |
| Immunogen Sequence | LYPYIVEFEEEAKNPGLETH RKRKRSGNSDSIERDAAQEA EAGTGLEPGSNSGQCSVPLK KGKDAPIKKESLGHWSQGLK ISMQDPKMQVYKDEQVVVIK DKYPKARYHWLVLPWTSISS LKAVAREHLELLKHMHTVGE KVIVDFAGSSKLRFRLGYHA IPSMSHVHLHVISQDFDSPC LKNKKHWNSFNTEYFLESQA VIEMVQEAGRVTVRDGMPEL LKLPLRCHECQQLLPSIPQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1). |
Additional Info
| Tissue Specificity | Widely expressed.detected in liver, kidney and lymph node (at protein level). Isoform 1 is highly expressed in the cerebral cortex and cerebellum, compared to isoform 2 (at protein level). Widely expressed.detected throughout the brain, in liver, kidney, skeletal muscle, fibroblasts, lymphocytes and pancreas. |
| Function | DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA). |
| Protein Name | AprataxinForkhead-Associated Domain Histidine Triad-Like ProteinFha-Hit |
| Database Links | |
| Cellular Localisation | NucleusNucleoplasmNucleolusUpon Genotoxic StressColocalizes With Xrcc1 At Sites Of Dna DamageColocalizes With Mdc1 At Sites Of Dna Double-Strand BreaksInteraction With Ncl Is Required For Nucleolar LocalizationIsoform 12: Cytoplasm |
| Alternative Antibody Names | Anti-Aprataxin antibodyAnti-Forkhead-Associated Domain Histidine Triad-Like Protein antibodyAnti-Fha-Hit antibodyAnti-APTX antibodyAnti-AXA1 antibody |
Information sourced from Uniprot.org