• Western blot analysis of lysates from wild type (WT) and 293T cells transfected with APOBEC3A using APOBEC3A Rabbit polyclonal antibody (STJ114275) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-APOBEC3A antibody (1-199) (STJ114275)

SKU:
STJ114275

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOBEC3A (1-199) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOBEC3A
Gene ID: 100913187/200315
Uniprot ID: ABC3A_HUMAN
Immunogen Region: 1-199
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human APOBEC3A (NP_663745.1).
Immunogen Sequence: MEASPASGPRHLMDPHIFTS NFNNGIGRHKTYLCYEVERL DNGTSVKMDQHRGFLHNQAK NLLCGFYGRHAELRFLDLVP SLQLDPAQIYRVTWFISWSP CFSWGCAGEVRAFLQENTHV RLRIFAARIYDYDPLYKEAL QMLRDAGAQVSIMTYDEFKH CWDTFVDHQGCPFQPWDGLD EHSQALSGRLRAILQNQGN
Tissue Specificity Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia.
Function DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic hypermutation in the nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
Protein Name Dna Dc->Du-Editing Enzyme Apobec-3a
A3a
Phorbolin-1
Database Links Reactome: R-HSA-72200
Reactome: R-HSA-75094
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Dna Dc->Du-Editing Enzyme Apobec-3a antibody
Anti-A3a antibody
Anti-Phorbolin-1 antibody
Anti-APOBEC3A antibody

Information sourced from Uniprot.org

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