• Western blot analysis of extracts of various cell lines, using APOA4 antibody (STJ111834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of HepG2 cells, using APOA4 antibody (STJ111834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of HepG2 cells, using APOA4 antibody (STJ111834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using APOA4 antibody (STJ111834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-APOA4 antibody (227-396) (STJ111834)

SKU:
STJ111834

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOA4 (227-396) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOA4
Gene ID: 337
Uniprot ID: APOA4_HUMAN
Immunogen Region: 227-396
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 227-396 of human APOA4 (NP_000473.2).
Immunogen Sequence: AQDTQEKLNHQLEGLTFQMK KNAEELKARISASAEELRQR LAPLAEDVRGNLRGNTEGLQ KSLAELGGHLDQQVEEFRRR VEPYGENFNKALVQQMEQLR QKLGPHAGDVEGHLSFLEKD LRDKVNSFFSTFKEKESQDK TLSLPELEQQQEQQQEQQQE QVQMLAPLES
Tissue Specificity Synthesized primarily in the intestine and secreted in plasma.
Post Translational Modifications Phosphorylation sites are present in the extracellular medium.
Function May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II.potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Protein Name Apolipoprotein A-Iv
Apo-Aiv
Apoa-Iv
Apolipoprotein A4
Database Links Reactome: R-HSA-8963888
Reactome: R-HSA-8963889
Reactome: R-HSA-8963901
Reactome: R-HSA-975634
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative Antibody Names Anti-Apolipoprotein A-Iv antibody
Anti-Apo-Aiv antibody
Anti-Apoa-Iv antibody
Anti-Apolipoprotein A4 antibody
Anti-APOA4 antibody

Information sourced from Uniprot.org

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