| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Aprataxin and PNK-like factor (82-131 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
APLF |
| Gene ID: |
200558 |
| Uniprot ID: |
APLF_HUMAN |
| Immunogen Region: |
82-131 aa |
| Specificity: |
APLF Polyclonal Antibody detects endogenous levels of APLF protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human APLF at the amino acid range 82-131 |
| Function | Histone chaperone involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with branched poly-ADP-ribose chains, a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Following recruitment to DNA damage sites, acts as a histone chaperone that mediates histone eviction during DNA repair and promotes recruitment of histone variant MACROH2A1. Also has a nuclease activity: displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). Also acts as a negative regulator of cell pluripotency by promoting histone exchange. Required for the embryo implantation during the epithelial to mesenchymal transition in females. |
| Protein Name | Aprataxin And Pnk-Like FactorApurinic-Apyrimidinic Endonuclease AplfPnk And Aptx-Like Fha Domain-Containing ProteinXrcc1-Interacting Protein 1 |
| Cellular Localisation | NucleusChromosomeCytoplasmCytosolLocalizes To Dna Damage SitesAccumulates At Single-Strand Breaks And Double-Strand Breaks Via The Pbz-Type Zinc Fingers |
| Alternative Antibody Names | Anti-Aprataxin And Pnk-Like Factor antibodyAnti-Apurinic-Apyrimidinic Endonuclease Aplf antibodyAnti-Pnk And Aptx-Like Fha Domain-Containing Protein antibodyAnti-Xrcc1-Interacting Protein 1 antibodyAnti-APLF antibodyAnti-C2orf13 antibodyAnti-PALF antibodyAnti-XIP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
