• Western blot analysis of various lysates, using Annexin A5/Annexin V antibody (STJ115880) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded Human colon using Annexin A5/Annexin V antibody (STJ115880) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using Annexin A5/Annexin V antibody (STJ115880) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human tonsil using Annexin A5/Annexin V antibody (STJ115880) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ANXA5 antibody (1-320) (STJ115880)

SKU:
STJ115880

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANXA5 (1-320) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANXA5
Gene ID: 308
Uniprot ID: ANXA5_HUMAN
Immunogen Region: 1-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human Annexin A5/Annexin V (NP_001145.1).
Immunogen Sequence: MAQVLRGTVTDFPGFDERAD AETLRKAMKGLGTDEESILT LLTSRSNAQRQEISAAFKTL FGRDLLDDLKSELTGKFEKL IVALMKPSRLYDAYELKHAL KGAGTNEKVLTEIIASRTPE ELRAIKQVYEEEYGSSLEDD VVGDTSGYYQRMLVVLLQAN RDPDAGIDEAQVEQDAQALF QAGELKWGTDEEKFITIFGT RSVSHLRKVFDKYMTISGFQ IEETIDRETSGNLEQLLLA
Post Translational Modifications S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Protein Name Annexin A5
Anchorin Cii
Annexin V
Annexin-5
Calphobindin I
Cpb-I
Endonexin Ii
Lipocortin V
Placental Anticoagulant Protein 4
Pp4
Placental Anticoagulant Protein I
Pap-I
Thromboplastin Inhibitor
Vascular Anticoagulant-Alpha
Vac-Alpha
Database Links Reactome: R-HSA-114608
Alternative Antibody Names Anti-Annexin A5 antibody
Anti-Anchorin Cii antibody
Anti-Annexin V antibody
Anti-Annexin-5 antibody
Anti-Calphobindin I antibody
Anti-Cpb-I antibody
Anti-Endonexin Ii antibody
Anti-Lipocortin V antibody
Anti-Placental Anticoagulant Protein 4 antibody
Anti-Pp4 antibody
Anti-Placental Anticoagulant Protein I antibody
Anti-Pap-I antibody
Anti-Thromboplastin Inhibitor antibody
Anti-Vascular Anticoagulant-Alpha antibody
Anti-Vac-Alpha antibody
Anti-ANXA5 antibody
Anti-ANX5 antibody
Anti-ENX2 antibody
Anti-PP4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance