• Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g Annexin A11 antibody (STJ29559). Western blot was performed from the immunoprecipitate using Annexin A11 antibody (STJ29559) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using Annexin A11 Rabbit polyclonal antibody (STJ29559) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using Annexin A11 Rabbit polyclonal antibody (STJ29559) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat heart, using Annexin A11 Rabbit polyclonal antibody (STJ29559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ANXA11 antibody (276-505) (STJ29559)

SKU:
STJ29559

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANXA11
Gene ID: 311
Uniprot ID: ANX11_HUMAN
Immunogen Region: 276-505
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 276-505 of human Annexin A11 (NP_001148.1).
Immunogen Sequence: FDIYEIKEAIKGVGTDEACL IEILASRSNEHIRELNRAYK AEFKKTLEEAIRSDTSGHFQ RLLISLSQGNRDESTNVDMS LAQRDAQELYAAGENRLGTD ESKFNAVLCSRSRAHLVAVF NEYQRMTGRDIEKSICREMS GDLEEGMLAVVKCLKNTPAF FAERLNKAMRGAGTKDRTLI RIMVSRSETDLLDIRSEYKR MYGKSLYHDISGDTSGDYRK ILLKICGGND
Function Binds specifically to calcyclin in a calcium-dependent manner. Required for midbody formation and completion of the terminal phase of cytokinesis.
Protein Name Annexin A11
56 Kda Autoantigen
Annexin Xi
Annexin-11
Calcyclin-Associated Annexin 50
Cap-50
Cellular Localisation Cytoplasm
Melanosome
Nucleus Envelope
Nucleus
Nucleoplasm
Cytoskeleton
Spindle
Found Throughout The Nucleoplasm At Interphase And During Mitosis Concentrates Around The Mitotic Apparatus
Elevation Of Intracellular Calcium Causes Relocalization From The Nucleoplasm To The Nuclear Envelope
With Little Effect On The Cytoplasmic Pool
Localization To The Nuclear Envelope Is Cell-Cycle Dependent
Alternative Antibody Names Anti-Annexin A11 antibody
Anti-56 Kda Autoantigen antibody
Anti-Annexin Xi antibody
Anti-Annexin-11 antibody
Anti-Calcyclin-Associated Annexin 50 antibody
Anti-Cap-50 antibody
Anti-ANXA11 antibody
Anti-ANX11 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance