| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ANO1 (400-500) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000IF/ICC 1:100-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ANO1 |
| Gene ID: |
55107 |
| Uniprot ID: |
ANO1_HUMAN |
| Immunogen Region: |
400-500 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human ANO1 (NP_060513.5). |
| Immunogen Sequence: |
ASHLFDNPATVFFSVFMALW AATFMEHWKRKQMRLNYRWD LTGFEEEEEAVKDHPRAEYE ARVLEKSLKKESRNKEKRRH IPEESTNKWKQRVKTAMAGV K |
| Tissue Specificity | Expressed in nasal epithelial cells (at protein level). In the kidney, expressed in the collecting duct (at protein level). Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles. Expressed in eccrine sweat glands. |
| Function | Calcium-activated chloride channel (CaCC). Plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. Required for CFTR activation by enhancing endoplasmic reticulum Ca(2+) store release and is also required for CFTR membrane expression. Required for basal and ATP-dependent mucus secretion in airways and intestine, probably by controlling exocytosis of mucus-filled granules by providing Ca(2+) to an apical signaling compartment. Contributes to airway mucus expression induced by interleukins IL3 and IL8 and by the asthma-associated protein CLCA1 and is required for expression of mucin MUC5AC. However, was shown in another study not to be required for MUC5AC expression. Plays a role in the propagation of Ca(2+) waves in Kolliker's organ in the cochlea and contributes to the refinement of auditory brainstem circuitries prior to hearing onset. In vomeronasal sensory neurons, modulates spontaneous firing patterns in the absence of stimuli as well as the firing pattern of pheromone-evoked activity. Responsible for calcium-activated chloride channel activity in type I taste cells of the vallate papillae. Acts as a heat sensor in nociceptive neurons. In dorsal root ganglion neurons, plays a role in mediating non-histaminergic Mas-related G-protein coupled receptor (MRGPR)-dependent itching, acting as a downstream effector of MRGPRs. In the developing brain, required for the Ca(2+)-dependent process extension of radial glial cells. Isoform 4: Calcium-activated chloride channel (CaCC). Contributes to calcium-activated chloride secretion in human sweat gland epithelial cells. Shows increased basal chloride permeability and decreased Ca(2+)-induced chloride permeability. Isoform 5: Calcium-activated chloride channel (CaCC). Shows increased sensitivity to intracellular Ca(2+). |
| Protein Name | Anoctamin-1Discovered On Gastrointestinal Stromal Tumors Protein 1Oral Cancer Overexpressed Protein 2Transmembrane Protein 16aTumor-Amplified And Overexpressed Sequence 2 |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-9733458 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinPresynapseIn Differentiating Airway Epithelial CellsPredominantly Intracellular At Day 0 But Is Apically Localized By Day 30Expressed In The Presynapse Of Retinal Neurons |
| Alternative Antibody Names | Anti-Anoctamin-1 antibodyAnti-Discovered On Gastrointestinal Stromal Tumors Protein 1 antibodyAnti-Oral Cancer Overexpressed Protein 2 antibodyAnti-Transmembrane Protein 16a antibodyAnti-Tumor-Amplified And Overexpressed Sequence 2 antibodyAnti-ANO1 antibodyAnti-DOG1 antibodyAnti-ORAOV2 antibodyAnti-TAOS2 antibodyAnti-TMEM16A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
